By serving as the auxiliary reducer in complex substrates, formate is an intermediate in the energy-saving pathway in complex environments (Ferry, 1990). ; Martn-Gil, F.J. (October 2004). Electrochemical activity of an Fe (III)-reducing bacterium. Shewanella putrefaciens (Derby and Hammer) MacDonell and Colwell - ATCC If you wish to become an apiweb user click on the following button : Registration. Vignier, Nicolas; Thodose, Rafaelle; Barreau, Morgane; Baubion, Emilie; Olive, Claude; Cabi, Andr; Hochedez, Patrick (10 July 2013). (I) NMR ownership figure of metabolites from S. putrefaciens. As a step in the development of . The sequences of these 12 differentially expressed proteins were aligned with sequences downloaded from NCBI. >1, upregulation; <1, downregulation; , no detect. W, water group (control); D, sewage group (experimental) (**P<0.01). It is the only nonfermentative gram-negative rod pathogens that produces hydrogen sulfide. This option allows users to search by Publication, Volume and Page Selecting this option will search the current publication in context. Wang L, Li Y, Yang XY, Zhang BB, Ninane N, Busscher HJ, Hu ZY, Delneuville C, Jiang N, Xie H, Van Tendeloo G, Hasan T, Su BL. XS proposed the study and provided guidance to all co-authors. The amino acid sequences of the differentially expressed proteins (detected by 2-DE) were downloaded from NCBI GenBank. S. putrefaciens is also a facultative anaerobe with the ability to reduce iron and manganese metabolically; that is, it can use iron and manganese as the terminal electron acceptor in the electron transport . Meat Refrigeration [PDF] [46iu2rhrjc70] Total RNA was extracted using 1-mL Trizol followed by a M-MuLV first strand cDNA synthesis kit (Sangon Biotech, Shanghai) to reverse transcribe RNA. An official website of the United States government. The non-interference protein assay kit SK3071 (Sangon Biotech, Shanghai) was used to quantify bacterial proteins using bovine serum protein as standards. Opportunistic infection of chronic skin ulcers with, Rupture of a primary aortic aneurysm infected with, Soft tissue infection and bacteremia caused by. This means that Shewanella infections occur in warm climates or during especially warm summers in temperate climates. The plates were incubated at 37 C overnight. sharing sensitive information, make sure youre on a federal Outer membrane proteins are the main component in the outer membrane of Gram-negative bacteria. A Comparison of Molecular Biology Mechanism of Shewanella putrefaciens Pinchuk G. E., Geydebrekht O. V., Hill E. A., Reed J. L., Konopka A. E., Beliaev A. S., et al. Shewanella (Pseudomonas) putrefaciens bacteraemia. The corresponding protein concentration was approximately 0.81.0g/L, which was the loading concentration of 2-DE sample (Figure (Figure22I). The present study aimed (i) to assess the relationship between c . Sh. Shewanella algae and Shewanella putrefaciens: clinical and - PubMed PMC The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. ABSTRACT The occurrence of the two Shewanella species found in clinical specimens, Shewanella algae and Shewanella putrefaciens, correlates with the temperature and salinity of seawater. As seen from the results of 2-DE and RT-qPCR, the upregulated expressions of Omp35, deoxyribose-phosphate aldolase, and OmpW in proteomics analysis were not verified by RT-qPCR. Before the genome of Shewanella was sequenced, it was thought to use a restricted range of substrates as carbon and energy sources (formate, lactate, and pyruvate). Therefore, in this study, we applied a combination of differential proteomics, metabolomics, and real-time fluorescent quantitative PCR techniques to identify bacteria intracellular metabolites. It is also one of the organisms associated with the odor of rotting fish, as it is a marine organism which produces trimethylamine (hence the species name putrefaciens, from putrid). We also investigated the carbohydrate metabolic pathway to understand S. putrefaciens colonization in sewage. -, Antimicrob Agents Chemother. Subcellular Min oscillations as a single-cell reporter of the action of polycations, protamine, and gentamicin on Escherichia coli. As a result, many rivers and oceans are contaminated. Generating an ePub file may take a long time, please be patient. Min, Maozhong; Xu, Huifang; Chen, Jia; Fayek, Mostafa (July 2005). The infections described most commonly involve ears, skin and soft tissue, with or without bacteraemia. The protocol isoelectric focusing of protein samples. MeSH Genome sequence of the dissimilatory metal ion-reducing bacterium. The genus Shewanella consists of Gram-negative proteobacteria that are ubiquitously distributed in environment. FOIA The strips were taken out from the refrigerator at 20C and placed at room temperature for 1h. The processed samples were put into the swelling tank, which was covered by the strips with the addition of mineral oil. Colony morphology of Shewanella strains on different plates. Figure 4a Reexamination of tetrodotoxin production by bacteria. J Appl Microbiol. Benedetti M., Giuliani M. E., Regoli F. (2015). In both solid and liquid media, S. putrefaciens is often recognizable by its bright pink color. D, fresh water; W, sewage. S. putrefaciens is one of several species that have been shown to derive energy by reducing U(VI) to U(IV), which is thought to be important in making Uranium deposits. The results of total volatile basic nitrogen (TVB-N . A polyphasic approach in typing of Shewanella spp. Prest E., El-Chakhtoura J., Hammes F., Saikaly P., Van Loosdrecht M., Vrouwenvelder J. The implication of these plasmids in the probiotic or pathogenic nature of S. putrefaciens is discussed. Rate the pronunciation difficulty of Shewanella Putrefaciens. The specific primers were designed and synthesized (Table S2 in Supplementary Material) for qRT-PCR. Vogel BF. Electrically conductive bacterial nanowires produced by, Systems analysis of a quorum sensing network: design constraints imposed by the functional requirements, network topology and kinetic constants, Microbiological spoilage of fish and fish products. Lower S. K., Hochella M. F., Beveridge T. J. Shewanella putrefaciens was isolated from sewage outlets and preserved at our laboratory (Figure S4 in Supplementary Material). Shewanella can be recovered from a variety of water sources, natural gas and petroleum reserves, dairy products, meat, and fish. World J Microbiol Biotechnol. Published by Elsevier Inc. We use cookies to help provide and enhance our service and tailor content. JX prepared other figures. The occurrence of the two Shewanella species found in clinical specimens, Shewanella algae and Shewanella putrefaciens, correlates with the temperature and salinity of seawater. On the contrary, the concentration of ethanolamine increased. Protein score is 10log(P), where P is the probability that the observed match is a random event. Regulation of colony morphology and biofilm formation in Shewanella algae Multi-omics based changes in response to cadmium toxicity in, The Colonization Mechanism of Shewanella putrefaciens in Marine Land-Based Sewage. The TCA cycle is a principal route of carbon metabolism during aerobic growth. Khalid A., Arshad M., Crowley D. E. (2008). The 2011 monitoring results of terrestrial sewage outlets into the sea in Ningbo City showed that COD, TP, and TN of Xiangshans Qiangtou comprehensive Sewage Outlet severely exceeded the standards. Shewanella - Wikipedia It is the only nonfermentative gram-negative rod pathogens that produces hydrogen sulfide. 1997 Aug;25(2):225-9. doi: 10.1086/514537. There are many reports that have reached similar conclusions. Production and specificity of poly and monoclonal antibodies raised against, The most obvious source for human infection is exposure to seawater. Wu X.-B., Tian L.-H., Zou H.-J., Wang C.-Y., Yu Z.-Q., Tang C.-H., et al. Zhang D., Xu J., He W., Tong Q., Chen L., Zhou J., et al. We found S. putrefaciens had 12 different proteins differentially expressed in freshwater culture than when grown in wastewater, referring to the formation of biological membranes (Omp35, OmpW), energy metabolism (SOD, deoxyribose-phosphate pyrophosphokinase), fatty acid metabolism (beta-ketoacyl synthase), secondary metabolism, TCA cycle, lysine degradation (2-oxoglutarate reductase), and propionic acid metabolism (succinyl coenzyme A synthetase). S. putrefaciens was grown in freshwater (W) as the control group (peptone 1%, beef extract 0.5%, NaCl 0.5%, deionized water) and sewage water (D) as the experiment group (peptone 1%, beef extract 0.5%). putrefaciens.[3][4][5]. Deoxyribose-phosphate aldolase (P2) showed the largest increase, with approximately fivefold increase in expression, followed by outer membrane protein OmpW (P3, 3.67), outer membrane porin Omp35 (P1, 3.28), and beta-ketoacyl synthase (P8, 3.18). According to the KEGG pathway, FDH is associated with the two-component signal transduction system (TCS), which is present in most bacteria for signal transduction. This study aimed to investigate the potential spoilage factors of S. putrefaciens by comparative proteomic analysis. Shewanella putrefaciens play a predominant role in food spoilage processes, mainly correlated with spoilage of frozen fish and meat products. 1,2, Cheng Guo. soured produced water, oil, limited oxygen environments) plays an important role in microbial activities leading to microbiologically influenced corrosion (MIC). Compared to the freshwater environment, the intracellular metabolite of S. putrefaciens grown in sewage is more complex in composition, with an increase in multiple metabolites. With the upregulation of formaldehyde (FDH), formate is oxidized to produce more NADH, which provides more energy to the bacteria growing under sewage stress and reduces the harm brought by toxic substances. Bloodstream Infections in Queensland, Australia", https://handwiki.org/wiki/index.php?title=Biology:Shewanella_putrefaciens&oldid=2294927. This protein is necessary for the normal growth of bacteria in such environments. Shewanella algae and Shewanella putrefaciens: clinical and As with all Shewanella, this organism produces hydrogen sulfide on TSI. Epub 2008 Oct 15. Huson D. H., Auch A. F., Qi J., Schuster S. C. (2007). For instance, Evguenieva-Hackenberg and Klug (2011) believed that in bacteria and archaebacteria, genomics results revealed the important role of posttranscriptional regulation in protein synthesis, as well as the low correlation between the expression on protein level and mRNA level. Pagani, Leonardo; Lang, Adolf; Vedovelli, Claudio; Moling, Oswald; Rimenti, Giovanni; Prister, Raffaele; Mian, Peter (May 2003). colonies were stored at -80. thermosphacta is unable to grow anaerobically below pH 5.8 (Gill, 1983). A representative of the dominant isolates, temporarily named Y2, was characterized further in the present . The chemical environment (e.g. Then, NMR spectrometer (Bruker AV III 600MHz spectrometer, equipped with a reverse probe) was used for mass spectrometry. Changes in cell morphology of Listeria monocytogenes and Shewanella Abstract As natural polyphenols have been known to have the deodorizing activity, the deodorizing properties and mechanisms of action of polyphenols, the main constituents of green tea extract (GTE), black tea extract (BTE), and grape seed extract (GSE), against volatile sulfur compounds (VSCs) in kimchi were investigated. Clipboard, Search History, and several other advanced features are temporarily unavailable. The temperatures of hydration and IEF were both set at 20C. Comparing S. putrefaciens growth in freshwater and sewage medium, 11 proteins were upregulated expression and 1 protein (SOD) was downregulated. Rewiring of transcriptional regulatory networks in the carbohydrate mechanisms of Shewanella which colonized in the sewage according to KEGG and previous research (Scott and Nealson, 1994; Fredrickson et al., 2008; Pinchuk et al., 2011). Metals also affect the structure, morphology, function, and activity of microorganisms in soils and substrates. (2014). The following preparation of membrane protein procedures was based on a method that we used previously (Weina, 2015; Zhang et al., 2015). The mouth of each tube was covered by thin film, which was pierced by a syringe to make small holes. Map of 10 terrestrial sewage outlets sites in coastal areas of Ningbo. We wish to thank Lauren Harroff for proofreading and the owner of the boat for supporting convenience to take samples. Atomic force microscopy revealed that protamine smoothed the surface of cells, formed holes in the cell envelope, and caused fusion of S. putrefaciens cells. Some researchers also reported the ability of Shewanella to treat azo dye waste water and analyzed the mechanism byproducts (Pearce et al., 2006; Khalid et al., 2008; Liu et al., 2013; Yu et al., 2015). The major cause of reservoir souring and subsequent in peritonitis patients by Raman spectroscopy, PHENOTYPIC CHARACTERISATION AND IDENTIFICATION. Gene expressions were detected via SYBR green quantitative PCR of 20L reactions with 2L cDNA, 0.8L forward and reverse primers, 10L SYBR II, and 6.4L molecular grade water. 2,3,4 . Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from, A biochemical study of the intermediary carbon metabolism of. The samples were combined, with triplicates for each group. With metabolomics, a biological system can be analyzed dynamically (Nicholson et al., 1999). Such an explosive proliferation might be related to the quorum-sensing function of bacteria (Goryachev et al., 2006). Before 2005. When colonies were visualized against a black background (referred to as "top-black view"), the Kim B. H., Ikeda T., Park H. S., Kim H. J., Hyun M. S., Kano K., et al. Shewanella putrefaciens, a sapro-phytic gram-negative rod, is rarely responsible for clinical syndromes in humans. The results showed that formate, ethanolamine, uracil, acetate, and putrescine scored highly. The .gov means its official. To obtain a positive result on the catalase test, scratch the surface of the colonies . 2014 Mar;30(3):1053-62. doi: 10.1007/s11274-013-1524-9. The catalase reaction is a weak positive. Study with Quizlet and memorize flashcards containing terms like What organism genuses are categorized as nonfermentative GNRs that are glucose oxidizers, oxidase positive, and can grow on Mac, Pseudomonas species of interest, Burkholderia species of interest and more. To update your cookie settings, please visit the, Guidelines, Position, and Consensus Papers, Cutaneous leishmaniasis: an increasing threat for travellers, Rapid identification of Candida spp. Clinical findings 1School of Marine Science, Ningbo University, Ningbo, China, 2College of Engineering, China Agricultural University, Beijing, China, 3Department of Food Science, Cornell University, Ithaca, NY, USA. The energy is supplied for the bacteria to enable growth in the sewage outlets. The authors also propose the carbohydrate mechanism for the colonization of sewage by S. putrefaciens (Figure (Figure44). The intracellular metabolites of S. putrefaciens grown in sewage and freshwater environment were extracted for NMR analysis. Shewanella putrefaciens - Missouri S&T Frontiers | Comparative Proteome Analysis of Shewanella putrefaciens Comparative Proteomics Reveals the Spoilage-Related Factors of 0. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Gorby Y. Total RNA and cDNA concentration were measured by Nano Drop 2000 (Thermo Scientific, MA, USA). Iran J Basic Med Sci. Johansen C, Verheul A, Gram L, Gill T, Abee T. Appl Environ Microbiol. Within the genus Shewanella, S. oneidensis MR-1 is a widely used genetic and physiological model with readily accessible gene manipulation strategies, a broad repertoire of anaerobic respiration pathways including metal oxide reduction, and diverse extracellular electron transfer capacities (Hau and Gralnick, 2007 ). A., Yanina S., Mclean J. S., Rosso K. M., Moyles D., Dohnalkova A., et al. Bacterial recognition of mineral surfaces: nanoscale interactions between, The outer membrane protein Omp35 affects the reduction of Fe (III), nitrate, and fumarate by, Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. Bhandari S, Pan TL, Horvath J, Tiller D.CAPD, swimming in Shewanella. The functionality is limited to basic scrolling. Pathogenic strains of Shewanella putrefaciens contain plasmids that are Shewanella putrefaciens is a gram-negative bacterium. Bacteriology of butter. The functions of the proteins were also queried (Sun et al., 2015). apiweb Go. found that the microbial growth, TVB-N content, and K value of fish inoculated with S. putrefaciens increased faster than the control. It has been demonstrated that the outer membrane proteins play an important role in the formation of biofilm. D, fresh water; W, sewage. Regulation of colony morphology and biofilm formation in Shewanella The specific parameters were as follows: temperature 298K, NMR frequency 600.13MHz, sampling times 128, sample delay 1, frequency domain 65,536, spectral width 12,019.231, number of sampling points 32,768, and pulse sequence noesypr1d/noesygppr1d. -, Proc Natl Acad Sci U S A. Microorganisms | Free Full-Text | Investigation of Lipolytic-Secreting (2015). Contaminated marine woundsthe risk of acquiring acute bacterial infection from marine recreational beaches. The remaining bacteria culture was stored at 4C. Identification was initially performed PDF Shewanella putrefaciens: an emerging pathogen for farmed yellow catfish Biomonitoring of metals under the water framework directive: detecting temporal trends and abrupt changes, in relation to the removal of pollution sources. They play an important role not only in substance transport, morphology maintenance, and synthesis of relevant substances but also in the adaptation of the organisms to the external environment (Lin et al., 2002). Shewanella putrefaciens belongs to the genus Shewanella, family Shewanellaceae, order Alteromonadales, class -Proteobacteria, and phylum Proteobacteria. 2008 Sep-Oct;225(1-3):27-37. doi: 10.1007/s00232-008-9130-8. Kim H. J., Park H. S., Hyun M. S., Chang I. S., Kim M., Kim B. H. (2002). I always wanted to know where the smell came from and now I know. Bacteriological studies on surface taint butter. and transmitted securely. Clin Infect Dis1995; 20: 13271332, Chen YS, Liu YC, Yen MY, Wang JH, Wang SR, Cheng DL. Book Search tips Selecting this option will search all publications across the Scitation platform Selecting this option will search all publications for the Publisher/Society in context It is also a facultative anaerobe with the ability to reduce iron and manganese metabolically which means it can use iron and manganese as the terminal electron acceptor in the electron transport chain. Skin and soft-tissue manifestations of, Shewanella putrefaciens infection. Small colonies of variant morphology became evident within 36 hours. Antigenic Characterisation of Tenacibaculum maritimum Isolates from Sea Bass (Dicentrarchus labrax, L.) Farmed on the Aegean Sea Coasts of Turkey As shown in Figure Figure2III,2III, 2 genes from S. putrefaciens were upregulated under sewage stress (P5: hypothetical protein; P8: beta-ketoacyl synthase), and 10 genes were downregulated. Differential expressions at the transcriptional level used protein score. This suggests that the expression of EF-Tu, whether gene or protein, was upregulated in S. putrefaciens extracted from sewage wastewater. After sequence alignment (Huson et al., 2007), the highly conserved regions were selected for primer design for quantitative PCR. This means that Shewanella infections occur in warm climates or during especially warm summers in temperate climates. Under static and low oxygen conditions, S.putrefaciens isolated from printing and dyeing sewage, such as the S. putrefaciens strain AS96, has a strong ability to treat dye-containing industrial effluents containing high concentrations of salt (Khalid et al., 2008). Biochemistry It is commonly found in water-related environments such as freshwater, seawater, lakes, rivers, sewage, fish, and soil (Bulut et al., 2004; Basir et al., 2012). already built in. Frontiers in Bioengineering and Biotechnology. The protein samples were serial diluted to 2-, 5-, 10-, and 50-fold.
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