The CCP4 suite: programs for protein crystallography. Meningococcal Disease John Wiley & Sons (1995). Acta Crystallogr. Burg, A. W. & Brown, G. M. The biosynthesis of folic acid VI. 6:6874 doi: 10.1038/ncomms7874 (2015). 2022 Springer Nature Switzerland AG. Its genome contains genes coding for three cytochrome P450s and two integral membrane alkane hydroxylases (AlkB1 & AlkB2), all assumed to perform hydroxylation of different linear or branched alkanes. All oligonucleotides were purchased from (Integrated DNA Technologies Inc., Coralville, IA) in a salt-free grade. The protein concentration was determined using the Bradford assay. It thrives in halophilic, aerobic environments and is found in the upper layers of freshwater or marine environments such as the Mediterranean Sea, Pacific Ocean and Arctic Sea [4, 8]. Monika Majersk - Associate Clinical Operations Specialist - LinkedIn 2019 Feb 18;19(1):41. doi: 10.1186/s12866-019-1406-x. Measurements were performed on a VP-Microcalorimeter (MicroCal, Northampton, MA) at 25C. Alcanivorax borkumensis are prominent actors in oil spill bioremediation; however, the interfacial dynamics of their biofilms and its role in oil degradation remain unclear. Alcanivorax borkumensis gen. nov., sp. Discover the world's research 20 . Alcanivorax borkumensis is a marine bacterium that uses exclusively petroleum oil hydrocarbons as sources of carbon and energy (and is therefore designated "hydrocarbonoclastic"). Microbes Environ. When needed, a final concentration of 5mM NaCl or KCl was also added at the same time. 1, 107125 (1999). The mechanism of a partial depolymerization of LDPE - a crucial step for its degradation by Alcanivorax borkumensis- is unknown and will be further investigated. J. Bacteriol. McCoy, A. J. et al. Commun. Terwilliger, T. C. Maximum-likelihood density modification using pattern recognition of structural motifs. USA 94, 1394413949 (1997). Calorimetry trials were also carried out in the absence of YdaH in the same experimental conditions. 2018 Dec 17;9:3130. doi: 10.3389/fmicb.2018.03130. It is found in low numbers in all oceans of the world and becomes abundant in oil-contaminated waters. Nat Commun 6, 6874 (2015). BMC Microbiol. 47, S216S224 (2008). Alcanivorax borkumensis is an oil-degrading marine bacterium. official website and that any information you provide is encrypted Structures of a Na+-coupled, substrate-bound MATE multidrug transporter. Prakash, S., Cooper, G., Singhi, S. & Saier, M. H. Jr. Various edge cases in . The Oil Eating Bacteria That Can Clean Up Crude Spills On the basis of the values of KA, the change in free energy (G) and entropy (S) were calculated with the equation: G=RT lnKa=HTS, where T is 273K and R is 1.9872, calK1 per mol. Science and Research Branch of Tehran, Islamic Azad University, Tehran, 1477893855, Iran, Faculty of Biological Sciences, Shahid Beheshti University, GC, Tehran, 19839, Iran, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, 14178, Iran, Genetics Department, Tarbiat Modares University, Tehran, 14115, Iran, Malek-Ashtar University of Technology, Tehran, 83145, Iran, You can also search for this author in J Biotechnol 106:215220, Hara A, Balik S, Syutsubo K, Misawa N, Smits THM, van Beilen JB, Harayama S (2004) Cloning and functional analysis of alkB genes in Alcanivorax Otwinowski, Z. MLPHARE, CCP4 Proc. Energy recovery Research Agency for funding under the scope of from secondary pulp/paper-mill sludge and sewage Cavitation as a Potential Technology for Wastewater Management - An Example of Enhanced Nutrient Release from Secondary Pulp and Paper Mill Sludge 647 Strojniki vestnik - Journal of Mechanical Engineering 65(2019)11-12, 641-649 . Bijan Bambai. : Alcanivorax borkumensis - Myhomeworkslave.com Alcanivorax strains represent one of the most studied group of n-alkane degraders able to produce biosurfactants. Pape, T. & Schneider, T. R. HKL2MAP: a graphical user interface for macromolecular phasing with SHELX programs. Bioassays 24, 637648 (2002). Enzymatic synthesis of dihydrofolic acid from guanine and ribose compounds. Article 187, 37133720 (2005). Sci. Frontiers | Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Bermingham, A. Nature 467, 484488 (2010). Alcanivorax borkumensis | SpringerLink Recently, a new class of glycolipids, glucose lipids, produced by Alcanivorax borkumensis SK2 ( Yakimov et al., 1998 ), has been described ( Abraham et al., 1998 ). Biotechnol. Environ Microbiol 6:191197, Harayama S, Kishira H, Kasai Y, Shutsubo K (1999) Petroleum biodegradation in marine environments. J. Mol. nov., members of the emended genus Alcanivorax. Biophys. Escherichia coli abg genes enable uptake and cleavage of the folate catabolite p-aminobenzoyl-glutamate. Chem. Below is the link to the electronic supplementary material. 2007 Jun;18(3):257-66. doi: 10.1016/j.copbio.2007.04.006. & Brown, G. M. The biosynthesis of folic acid. ISSN 2041-1723 (online). Chem. Natl Acad. Neutral lipid production in Alcanivorax borkumensis SK2 and other marine hydrocarbonoclastic bacteria . Wang, P., Read, M., Sims, P. F. G. & Hyde, J. E. Sulfadoxine resistance in the human malaria parasite Plasmodium falciparum is determined by mutations in dihydropteroate synthetase and an additional factor associated with folate utilization. Biol. The use of 10% (v/v) of inoculum concentration . 52, 30523060 (2008). DSM 11573. D Biol. Researchers have genecally engineered other bacteria to consume petroleum . Site-directed point mutations on residues D180, N390, W400, P418, R426, D429 and N433, which are expected to be critical for the function of the YdaH transporter, were performed to generate the single point mutants D180A, N390A, W400A, P418A, R426A, D429A and N433A. Oil Gas Sci Technol 58:427440, Article We show experimentally that biofilm dendrites emerge from aster-like nematic defects in the thin. This structure was then used to refine against the 2.96--resolution data using PHENIX41 as described above. Perspective Perspective: what is known, and not known, about the connections between alkane oxidation and metal uptake in alkanotrophs in the marine environment How to cite this article: Bolla, J.R. et al. place your first order and save 15% using coupon: The susceptibilities to sulfamethazine, sulfadiazine, sulfathiazole and sulfanilamide of E. coli BL21(DE3)abgTpabA harbouring pET15bydaH expressing the wild-type or mutant transporters, or the pET15b empty vector were tested on agar plates. Alcanivorax borkumensis. Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. and transmitted securely. Structure-based mechanism for Na+/melibiose symport by MelB. When possible, spill responders start mechanical recovery immediately, using booms, skimmers, and other equipment. Many organisms including Alcanivorax borkumensis (A. borkumensis) are capable of degrading petroleum oil as they rely on . Assess the spill and determine whether help is needed. Analysis of Storage Lipid Accumulation in Alcanivorax borkumensis Cartwright K. A. V. Gene diversity of CYP153A and AlkB alkane hydroxylases in oil-degrading Production of a recombinant alkane hydroxylase (AlkB2) from Alcanivorax Environ Microbiol 6:264273, Article Biochim. Alcanivorax borkumensis skyrocketed, helping to get rid of the oil. Alcanivorax borkumensis are prominent actors in oil spill bioremediation; however, the interfacial dynamics of their biofilms and its role in oil degradation remain unclear. Alcanivorax borkumensis is an oil-degrading marine bacterium. Adams, P. D. et al. Best Research Paper in Borneo Scientific Fair (National Level) "In Vitro Fertilization Using Embryonic-Stem cells to Increase Cattle Productivity . Alcanivorax borkumensis (A. borkumensis) is a Gram-negative, rod-shaped hydrocarbonoclastic ("oil-degrading") bacterium. Article Keith Perkins - PHD Candidate - Rutgers University | LinkedIn On the basis of the molecular weight of YdaH (51.6kDa), four molecules per asymmetric unit with a solvent content of 73.4% were expected. & Wanner, B. L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. By clicking accept or continuing to use the site, you agree to the terms outlined in our. PubMed The initial model was built manually using Coot39. D Biol. Lei, H. T., Bolla, J. R., Bishop, N. R., Su, C. C. & Yu, E. W. Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation. The RND permease superfamily: an ancient, ubiquitous and diverse family that includes human disease and development protein. ATCC 700651. J. Mol. and E.W.Y. Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. Crystallogr. In brief, E. coli BL21(DE3)abgTpabA carrying pET15bydaH or pET15b were grown in LB broth with 100gml1 ampicillin at 37C. PubMed Mol. Collaborative Computational Project No. Its ubiquity and unusual physiology suggest it is globally important in the removal of hydrocarbons from polluted marine systems. Moreover, Alcanivorax borkumensis is able to produce a glucolipid biosurfactant [14,15] which may enhance the bioavailability of hydrocarbons by either increasing apparent hydrocarbon solubility . J. Appl. Biotechnol Lett 32, 497502 (2010). Efran ManillaPrez and Alvin Brian Lange contributed equally to this work. An All-Oil Diet - The New York Times PLoS ONE 6, 15610 (2011). E-mail: esegal@technion.ac.il Its genome contains genes coding for three cytochrome P450s and two integral membrane alkane hydroxylases (AlkB1 & AlkB2), all assumed to perform hydroxylation of different linear or branched alkanes. . oil), polychlorinated biphenyls (PCBs), polyaromatic hydrocarbons (PAHs), heterocyclic compounds (such as pyridine or quinoline . - 212.48.68.247, Institute of Narcology Ministry of Health (3000601956). Attachment of Alcanivorax borkumensis to Hexadecane-In-Artificial Sea Water Emulsion Droplets. 2010 May;86(6):1693-706. doi: 10.1007/s00253-010-2515-5. Semi-finalist in Scientific Paper-Based Competition (National Level) "Bioremediasi Laut Menggunakan Alcanivorax borkumensis SK2 sebagai Solusi Masalah Pencemaran Limbah Industri" UNNES 2021 . Semantic Scholar is a free, AI-powered research tool for scientific literature, based at the Allen Institute for AI. Alcanivorax, first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not . Its ubiquity, unusual physiology and demonstrated role in . Richey, D. P. & Brown, G. M. The biosynthesis of folic acid. choo choo, 1941 crossword clue; blazor http get with parameters; best crispy french toast recipe; react form submit example; avant que subjunctive or indicative Commun. The protein sample was then adjusted to a final concentration of 20M. My research career began at Southern University at New Orleans when I became a work-study . The primers used for these mutations are listed in Supplementary Table 2. Use of the Advanced Photon Source is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract No. . How effective is in-situ burning? D Biol. This work is based on research conducted at the Northeastern Collaborative Access Team beamlines of the Advanced Photon Source, supported by an award GM103403 from the National Institutes of General Medical Sciences. Bacterial strains and plasmids used in this study are listed in Table Table1. PubMed Folster, J. P. & Shafer, W. M. Regulation of mtrF expression in Neisseria gonorrhoeae and its role in high-level antimicrobial resistance. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Schneiker, S. et al. When feasible to carry . wrote the paper. Acta Crystallogr. Daley, D. O. et al. Chemother.
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